GENPOS-SCONDB
Design conditional knock‑outs in 300+ vertebrate.
"Single injection, hundreds of species."
SCON (Short Conditional intrON) is a next-generation tool that radically simplifies functional genetic studies. With just a 189-bp sequence, SCON enables conditional intron functionality across various vertebrate species. A single insertion allows fast and powerful generation of conditional alleles — all without the complexity of traditional floxed systems.
SCON—a Short Conditional intrON for conditional knockout
Experimental & molecular medicine 2022
The generation of conditional alleles using CRISPR technology is still challenging. Here, we introduce a Short Conditional intrON (SCON, 189 bp) that enables the rapid generation of conditional alleles via one-step zygote injection...
- One-step zygote injection
- 13 mouse lines generated
- MAGR/VDGN site tagetable
Red2Flpe-SCON: Multicolor Conditional Knockout Mice
Nature Communications 2024
Red2Flpe-SCON: a mosaic knockout system that uses multicolor reporters to label both mutant and wild-type cells. This enables analysis of gene function at clonal resolution...
- Red-only Flpe expression system
- FRT-based SCON integration
- Used in Sox2 clonal analysis
Universal Conditional Knockout in Fish & Human iPSCs
2025
This study introduces a broadly applicable approach to generate conditional loss-of-function mutations in zebrafish, iPSCs, and organoids using a streamlined SCON system...
- Cross-species SCON compatibility
- Essential-gene-safe cassette
- Organoid & human iPSC tested
- Web-based knockout design tool
Database overview
Statistics
Release Note
2024.10.15
Draft version publish
2025.04.17
v1.0.0 First release